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While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. The Culture Collections represent deposits of cultures from world-wide sources.

Important technical information for successful culture of your cells.RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS). Cells grow slowly after resuscitation and subculture and may take 48-72 hours until exponential growth is established. Do not allow cells to become confluent, subculture at 75% to 90% confluency free-floating aggregates accumulate with time. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Maintain cultures between 3-9x100,000 cells/ml 5% CO2 37☌. Hyperlinks to MSDS documents:įrozen cell cultures Material Safety Data Sheet Growing cell cultures Material Safety Data Sheet Nucleic acids derived from cell cultures Material Safety Data Sheet ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP = Advisory Committee on Dangerous Pathogens (UK)Īll cell cultures have the potential to carry as yet unidentified adventitious agents. Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. The line is useful for studying soluble factors involved in B-cell differentiation.

If use of this culture results in a scientific publication, it should be cited in the publication as: BCL1 Clone CW13.20-3B3 (ECACC 90061904)ĭerived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. ECACC General Cell Collection: BCL1 Clone CW13.20-3B3Įuropean Collection of Authenticated Cell Cultures (ECACC)